Assay Features

methodology:

Chemiluminescense

specimen:

Serum

sample size:

10 uL

species:

Human

sensitivity:

1.52 ug/dL

range:

1.52 - 1020 ug/dL

analytic time:

2 Days

reporting options:

Web, E-Mail, Letter

Significance and Uses

Dehydroeprandrosterone (DHEA) is a steroid hormone produced by the adrenal glands, gonads, adipose tissue and the brain. DHEA is structurally similar to, and is a precursor of, testosterone and estrogen. In blood, most DHEA is found as its derivative dehydroeprandrosterone sulfate, DHEA-S. In humans, DHEA production peaks in early adulthood, around the age of 25, and declines afterwards to approximately 10% of peak levels by age 80. The significance of DHEA in health and disease is not fully established.

Reference Values

males:

80-560 ug/dL  

females premenopausal:

35-430 ug/dL  

females postmenopausal:

 

Assay Method

Dehydroepiandrosterone sulphate (DHEAS) is measured with an automated chemiluminescent assay using the Bayer Diagnostics ACS:180. (1) The chemiluminescent immunoassay involved the competitive binding of a Dimethylacridinium ester (DMAE) labeled DHEAS derivative to a rabbit anti-DHEAS antibody. The solid phase is Goat anti-rabbit IgG conugated to superparamagnetic particles (PMP). Closely related steroids show little cross reactivity in the assay indicating high specificity. The assay demonstrates acceptable parallelism of diluted serum samples and recovery of added exogenous hormone. DHEA cross reacts 192%, but the low levels of serum DHEA render this inconsequential. The intra assay coefficient of variation is 8.02% (n=261) and inter assay coefficient of variation is 11.34% (53.32 ug/dL, n=37) and 9.74% (250.21 ug/dL, n=37).

Typical Standard Curves

Ordering information

Catalog number:

A01_DHSSR_01

Priced from:

$0.00 (1) ($ USD): Per vial (data point). No charge for vehicle and positive controls.

(1) Price quoted assumes that: this assay is part of a larger panel of assays (minimum of 4 analytes) ordered for this sample; that the sample arrives properly aliquoted in a CLASS Laboratory bar coded sample vial; that the quantity of samples shipped to our facility is sufficient for batch processing of the samples and, that these samples will be subject to a single use-and-dispose cycle (i.e., no long term storage). Price does not include shipping. Other charges may also apply. Please contact us directly for a quote tailored to your study.